Difference between revisions of "Part:BBa K733009:Design"
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===Source=== | ===Source=== | ||
| − | We digest and ligate our [https://parts.igem.org/Part:BBa_K733001 | + | We digest and ligate our [https://parts.igem.org/Part:BBa_K733001 ''Ptms''] and [https://parts.igem.org/Part:BBa_E0240 BBa_E0240] from 2012 Kit Plate. |
| − | Backbone: | + | Backbone: ''Ptms'' in pSB1C3. Enzyme used: SpeI and PstI-HF. |
Insert: BBa_E0240. Enzyme used: XbaI and PstI-HF. Purified by gel purification. | Insert: BBa_E0240. Enzyme used: XbaI and PstI-HF. Purified by gel purification. | ||
===References=== | ===References=== | ||
Latest revision as of 16:28, 26 September 2012
Ptms+BBa_E0240: Ptms+RBS+GFP+Double
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 736
Design Notes
None.
Source
We digest and ligate our Ptms and BBa_E0240 from 2012 Kit Plate.
Backbone: Ptms in pSB1C3. Enzyme used: SpeI and PstI-HF.
Insert: BBa_E0240. Enzyme used: XbaI and PstI-HF. Purified by gel purification.