Difference between revisions of "Part:BBa K733009:Design"

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===Source===
 
===Source===
  
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Not available at this moment.
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We digest and ligate our [https://parts.igem.org/Part:BBa_K733001 ''Ptms''] and [https://parts.igem.org/Part:BBa_E0240 BBa_E0240] from 2012 Kit Plate.
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Backbone: ''Ptms'' in pSB1C3. Enzyme used: SpeI and PstI-HF.
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Insert: BBa_E0240. Enzyme used: XbaI and PstI-HF. Purified by gel purification.
  
 
===References===
 
===References===

Latest revision as of 16:28, 26 September 2012

Ptms+BBa_E0240: Ptms+RBS+GFP+Double


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 736


Design Notes

None.

Source

We digest and ligate our Ptms and BBa_E0240 from 2012 Kit Plate.

Backbone: Ptms in pSB1C3. Enzyme used: SpeI and PstI-HF.

Insert: BBa_E0240. Enzyme used: XbaI and PstI-HF. Purified by gel purification.

References