Difference between revisions of "Part:pSB1A10:Design"
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The process for building pSB1A10,was:
 
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#Build [[Part:BBa_I13534|I13534]] using normal BB construcion.
 
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#Insert [[Part:BBa_I13534|I13534]] into psb1A3 plasmid
 
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#Cap the BB prefix and suffix using [[Part:BBa_I13450|I13450]]
 
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#Re-insert a BB prefix/suffix into the center of [[Part:BBa_I13534|I13534]] using primers to the "insertion region" of [[Part:BBa_I13457|I13457]] and around-the-horn PCR.
 
  
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Some design notes:
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__NOTOC__
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#The inclusion of the 'barcode' was unintentional (it was attached to [[Part:BBa_E1010|E1010]]), it has no (intended) function.
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<partinfo>pSB1A10 short</partinfo>
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#The "spacer artifacts" at position 22 and 5225 are artifacts from around-the-horn PCR for inserting the BB restriction sites into the center of the plasmid.  If you look at [[Part:BBa_I13457|I13457]] you will see the corresponding regions in the section annotated "insertion region".
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#The RNAse E sites are intended to insulate the device being screened from the screening plasmid itself.  See a more detailed explanation [http://openwetware.org/wiki/Endy:Screening_plasmid_1.0 here].
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#The hairpin 5' to [[Part:BBa_E1010|E1010]] is meant to stabilize the mRNA transcript by preventing degredation by RNAse E. <site>keasling</site>
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#*however, there is a C deletion at bp 64 from the sequence used previously<cite>keasling</cite>.  For our current purposes the expression level of E1010 (mRFP1) has been sufficient with this mutated form of the hairpin, so we haven't made an effort to change it.
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==References==
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<partinfo>pSB1A10 SequenceAndFeatures</partinfo>
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<biblio>
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#keasling pmid=11097920
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</biblio>
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===Design Notes===
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===Source===
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===References===

Revision as of 02:17, 5 June 2006


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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.


Status: 500 Content-type: text/html

Software error:

Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
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BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.


Design Notes

Source

References