Difference between revisions of "Part:BBa K819005"

Revision as of 15:39, 26 September 2012

Constitutive LuxBrick Generator

It is a fusion protein consisting of E.coli endogenous SOS system repressor LexA and fungus N.crass light sensing protein VVD with LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and M135I. This part serves as an ultra sensitive photoreceptor(which can sense light as weak as moonlight) and will induce a light-dependent repression of genes under specific promoter in 408 form.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 3014
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2012
Illegal XhoI site found at 2842
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4401
Illegal BsaI.rc site found at 1410
Illegal SapI.rc site found at 4726

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-a fusion protein consisting of E.coli endogenous SOS system repressor LexA and fungus N.crass photosensor protein VVD with LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and M135I. This part serves as an ultra sensitive photoreceptor(which can sense light as weak as moonlight) and will induce a light-dependent repression of genes under specific promoter in 408 form.
+It is a fusion protein consisting of <i>E.coli</i> endogenous SOS system repressor LexA and fungus <i>N.crass</i> light sensing protein VVD with LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and M135I. This part serves as an ultra sensitive photoreceptor(which can sense light as weak as moonlight) and will induce a light-dependent repression of genes under specific promoter in 408 form.