Difference between revisions of "Part:BBa K805013:Design"

(References)
(Design Notes)
 
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===Design Notes===
 
===Design Notes===
test
+
sequence confirmed
 
+
 
+
  
 
===Source===
 
===Source===
  
yes
+
Extracted from the plasimd pEntr-3C
  
 
===References===
 
===References===
 
Hu LL, Zhang SS, Li XX, Wang BL.The use of the ccdB lethal gene for constructing a zero background vector in order to clone blunt-end PCR products.Mol Biol (Mosk). 2010 Jan-Feb;44(1):174-6.
 
Hu LL, Zhang SS, Li XX, Wang BL.The use of the ccdB lethal gene for constructing a zero background vector in order to clone blunt-end PCR products.Mol Biol (Mosk). 2010 Jan-Feb;44(1):174-6.

Latest revision as of 14:42, 26 September 2012

CcdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


Design Notes

sequence confirmed

Source

Extracted from the plasimd pEntr-3C

References

Hu LL, Zhang SS, Li XX, Wang BL.The use of the ccdB lethal gene for constructing a zero background vector in order to clone blunt-end PCR products.Mol Biol (Mosk). 2010 Jan-Feb;44(1):174-6.