Difference between revisions of "Part:BBa K805013:Design"

 
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===References===
 
===References===
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Hu LL, Zhang SS, Li XX, Wang BL.The use of the ccdB lethal gene for constructing a zero background vector in order to clone blunt-end PCR products.Mol Biol (Mosk). 2010 Jan-Feb;44(1):174-6.

Revision as of 14:32, 26 September 2012

CcdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


Design Notes

test


Source

yes

References

Hu LL, Zhang SS, Li XX, Wang BL.The use of the ccdB lethal gene for constructing a zero background vector in order to clone blunt-end PCR products.Mol Biol (Mosk). 2010 Jan-Feb;44(1):174-6.