Difference between revisions of "Part:BBa K863201:Design"
(→Design Notes) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K863201 short</partinfo> | <partinfo>BBa_K863201 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | primer | + | Primers for isolation of the sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer. <br> |
| − | + | fwd: 5'-ACGTgaattcgcggccgcttctagagCGAGTATCTATGATTGGAA-3' <br> | |
| − | + | rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTAAAAACAAGATAGTGCCCCTC-3' | |
===Source=== | ===Source=== | ||
Revision as of 14:29, 26 September 2012
3' UTR site of alcohol oxidase 1 gene (aox1)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 59
Design Notes
Primers for isolation of the sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTgaattcgcggccgcttctagagCGAGTATCTATGATTGGAA-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTAAAAACAAGATAGTGCCCCTC-3'
Source
The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR.