Difference between revisions of "Part:BBa K863200:Design"

(→‎Source)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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coming soon
+
Primers for isolation of the sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer. <br>
−
 
+
fwd: 5'-ACGTgaattcgcggccgcttctagagAGATCTAACATCCAAAGACG-3' <br>
−
 
+
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTACGTTTCGAATAATTAGTTGT-3'
  
 
===Source===
 
===Source===

Revision as of 14:27, 26 September 2012

5' UTR site of alcohol oxidase 1 gene (aox1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for isolation of the sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTgaattcgcggccgcttctagagAGATCTAACATCCAAAGACG-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTACGTTTCGAATAATTAGTTGT-3'

Source

Pichia pastoris

References