Difference between revisions of "Part:BBa K883500"
 
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<partinfo>BBa_K883500 short</partinfo>
 
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==TuYV coat protein==
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This plasmid contains the TuYV coat protein, which can be used as building block for TuYV VLPs.  
 
This plasmid contains the TuYV coat protein, which can be used as building block for TuYV VLPs.  
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Turnip Yellows Virus (TuYV) is member of the genus Polerovirus and family Luteoviridae, which is positive sense RNA virus. The viral gene encoding the coat protein for TuYV, (GenBank: NC_003743.1 (3483..5495)) was obtained from a plasmid encoding the entire viral genome (GenBank: X13063.1).  With designed primers, iGEM prefix and suffix were added to the 5 prime end and 3 prime end of the coat protein gene separately.
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Then the PCR products were digested, ligated to pSB1C3 backbone, and transformed into DH5 alpha competent E.coli cells. Because we used white and red screening, the white colonies, which are colonies with right inserts, were checked. They were first tested by colony PCR, after miniprep, they were examined by digestion check and send out for sequencing. All the tests confirmed that TuYV coat protein was inserted into the pSB1C3 vector.
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Latest revision as of 14:18, 26 September 2012

TuYV coat potein


This plasmid contains the TuYV coat protein, which can be used as building block for TuYV VLPs.


Turnip Yellows Virus (TuYV) is member of the genus Polerovirus and family Luteoviridae, which is positive sense RNA virus. The viral gene encoding the coat protein for TuYV, (GenBank: NC_003743.1 (3483..5495)) was obtained from a plasmid encoding the entire viral genome (GenBank: X13063.1). With designed primers, iGEM prefix and suffix were added to the 5 prime end and 3 prime end of the coat protein gene separately.


Then the PCR products were digested, ligated to pSB1C3 backbone, and transformed into DH5 alpha competent E.coli cells. Because we used white and red screening, the white colonies, which are colonies with right inserts, were checked. They were first tested by colony PCR, after miniprep, they were examined by digestion check and send out for sequencing. All the tests confirmed that TuYV coat protein was inserted into the pSB1C3 vector.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


*Safety notice*

This part is isolated from a virus and, when assembled, will form particles that very much resemble this virus in size and shape. However, no additional viral information is stored in this part and viral infection and/or replication can therefore be ruled out. It is completely safe for use in normal laboratory circumstances.