Difference between revisions of "Part:BBa K391006:Design"
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<partinfo>BBa_K391006 short</partinfo> | <partinfo>BBa_K391006 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We designed forward and reverse primers with the appropriate standard BioBrick restriction sites (<i>Eco</i>RI, <i>Xba</i>I, <i>Spe</i>I, <i>Pst</i>I) on each primer in order to put it on psb1C3. One out of two sets of primers included a histidine tag, which would be used to isolate DspB after it is expressed. | |
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===Source=== | ===Source=== | ||
Latest revision as of 14:03, 26 September 2012
DspB - carbohydrate digesting protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 967
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed forward and reverse primers with the appropriate standard BioBrick restriction sites (EcoRI, XbaI, SpeI, PstI) on each primer in order to put it on psb1C3. One out of two sets of primers included a histidine tag, which would be used to isolate DspB after it is expressed.
Source
The gene that encodes our protein, DspB, stems from the bacteria Actinobacillus actinomycetemcomitans.