Difference between revisions of "Part:BBa K823042"

Latest revision as of 14:02, 26 September 2012

PsspK promoter regulated by sigma G

A B. subtilis promoter regulated by σ G late in Sporulation in the forespore

Our team used this part in the combination with P_ydfG,ecf41_{Bli aa 1-204} and MazF in the [http://2012.igem.org/Team:LMU-Munich/Germination_Stop#Suicide_switch Suicide switch].

σ G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K823042 short</partinfo>
-A ''B. subtilis'' promoter regulated by sigma G late in Sporulation in the forespore
+A ''B. subtilis'' promoter regulated by σ G late in Sporulation in the forespore
 [[Image:SuicideSwitchgrafik.jpg|600px]]
@@ Line 10: / Line 10: @@
 σ G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination.
-<b>This Platereader measurment is the first and not reproduced to this point!</b>
-[[Image:PlatereaderPspoIVBecf41PydfG.jpg|600px]]
-This Platereader assay was done with the ''Bacillus subtilis'' strain W168 containing ''thrC''::P<sub>''spoIVB''</sub>-''ecf41<sub>Bli aa  1-204</sub>'' (through transformation with pSBBs4S-P<sub>''spoIVB''</sub>-''ecf41<sub>Bli aa  1-204</sub>'') and ''sacA''::P<sub>''ydfG''</sub>-''luxABCDE'' (through transformation with pSBBs3C-''luxABCDE''-P<sub>''ydfG''</sub>). That we get a signal shows that this is active together with [https://parts.igem.org/Part:BBa_K823041 P<sub>''ydfG''</sub>].