Difference between revisions of "Part:pSB1AK3"

Revision as of 02:17, 5 June 2006

High copy BioBrick assembly plasmid

pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Usage and Biology

Kanamycin resistant up to 5000 ug/ml.

Tetracycline sensitive at 1.5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3168
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3174
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3168
Illegal XhoI site found at 1035
Illegal XhoI site found at 2061
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 3168
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 3168
Plasmid lacks a suffix.
Illegal XbaI site found at 3183
Illegal SpeI site found at 2
Illegal PstI site found at 16
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2207

@@ Line 4: / Line 4: @@
 pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance.   The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
 ===Usage and Biology===
 Kanamycin resistant up to 5000 ug/ml.
@@ Line 14: / Line 16: @@
 <partinfo>pSB1AK3 SequenceAndFeatures</partinfo>
+<!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>pSB1AK3 parameters</partinfo>
+<!-- -->