Difference between revisions of "Part:BBa J09855:Experience"

Revision as of 11:53, 26 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J09855

We use this part to work as a promoter system which can be activated by C6HSL. We can add some other gene dowmstream so that AHL can work as a signal. In our project this year(Team: Tsinghua), we use this part and modified it to get more complicated construct.

User Reviews

UNIQ420d493ba41f8694-partinfo-00000000-QINU UNIQ420d493ba41f8694-partinfo-00000001-QINU To tell the truth, when we use this part for the first time, it didn't work well. Then we sequenced the part using prefix and suffix primer. We found that there are some part missed in Plac promoter at the beginning and in Plux promoter at the end. So we rebuilt this part and modified for a little. This modification improve the efficiency of transcription. The sequensing results show that our construct is correct.

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-To tell the truth, when we use this part for the first time, it didn't work well. Then we sequenced the part using prefix and suffix primer. We found that there are some part missed in Plac promoter at the beginning and in Plux promoter at the end. So we rebuild this part by ourselves and the sequensing results show that our construct is correct.
+To tell the truth, when we use this part for the first time, it didn't work well. Then we sequenced the part using prefix and suffix primer. We found that there are some part missed in Plac promoter at the beginning and in Plux promoter at the end. So we rebuilt this part and modified for a little. This modification improve the efficiency of transcription. The sequensing results show that our construct is correct.