Difference between revisions of "Part:BBa K819008"

Revision as of 10:03, 26 September 2012

Lux Operon under T7 promoter

Lux operon genes (from BBa_K325909) and related RBS are placed under T7 promoter. Cells transformed with this part can produce blue luminescence while no exogenous substrate is needed.

luxbrick under T7 promoter is very modular, because it is transcribed by T7 polymerase, which can be placed under any other promoter, forming a interface between luxbrick and other systems. Also, when transformed into BL21 cells, it can be induced with IPTG to reach a high expression level.

Usage and Biology

BL21 cells harboring T7-lux operon induced with IPTG at 5*10^-5M is shown in the photo
The best temperature for cells to glow is 30^oC rather than 37^oC.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 3014
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2012
Illegal XhoI site found at 2842
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4401
Illegal BsaI.rc site found at 1410
Illegal SapI.rc site found at 4726

Revision as of 09:27, 26 September 2012 (view source) Zhangzidong (Talk \| contribs) (→‎Usage and Biology) ← Older edit		Revision as of 10:03, 26 September 2012 (view source) Zhangzidong (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K819008 short</partinfo>		<partinfo>BBa_K819008 short</partinfo>

−	Lux operon genes (from [https://parts.igem.org/Part:BBa_K325909:Design BBa_K325909]) and related RBS are placed under T7 promoter. Cells transformed with this part can produce blue luminescence while no exogenous substrate is needed.<br/><br/>luxbrick under T7 promoter is very modular, because it is transcribed by T7 polymerase, which can be placed under any other promoter, forming a interface between luxbrick and other systems.Also, when transformed into BL21 cells, it can be induced with IPTG to reach a high expression level.	+	Lux operon genes (from [https://parts.igem.org/Part:BBa_K325909:Design BBa_K325909]) and related RBS are placed under T7 promoter. Cells transformed with this part can produce blue luminescence while no exogenous substrate is needed.<br/><br/>luxbrick under T7 promoter is very modular, because it is transcribed by T7 polymerase, which can be placed under any other promoter, forming a interface between luxbrick and other systems. Also, when transformed into BL21 cells, it can be induced with IPTG to reach a high expression level.