Difference between revisions of "Part:BBa K786003"

 
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<partinfo>BBa_K786003 short</partinfo>
 
<partinfo>BBa_K786003 short</partinfo>
  
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Sensory rhodopsin I fusion with HtrII & Tar, which is sensitive to orange light, with sensing spectra covering from 590-650 nm.
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Sensory Rhodopsin I have been fused with HtrI with the a linker peptide, only the membrane-proximal cytoplasmic domain have been kept, while the cytoplasmic domains have been replaced by that of Tar. Once the fusion protein was triggered, the histidine kinase (CheA) will be regulated, leading to phototactic effect, which is sensitive to orange light, with sensing spectra covering from 560-600 nm.
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We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
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We used it for sensing orange light to achieve phototaxis and switch for downstream gene expression.
  
 
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<!-- Add more about the biology of this part here

Revision as of 09:36, 26 September 2012

Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light

Sensory Rhodopsin I have been fused with HtrI with the a linker peptide, only the membrane-proximal cytoplasmic domain have been kept, while the cytoplasmic domains have been replaced by that of Tar. Once the fusion protein was triggered, the histidine kinase (CheA) will be regulated, leading to phototactic effect, which is sensitive to orange light, with sensing spectra covering from 560-600 nm. We used it for sensing orange light to achieve phototaxis and switch for downstream gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 785
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 37
    Illegal NgoMIV site found at 614
    Illegal NgoMIV site found at 638
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 875
    Illegal AgeI site found at 144
    Illegal AgeI site found at 1616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 977