Difference between revisions of "Part:BBa K819015:Design"
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===Design Notes===
 
===Design Notes===
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CheZ coding sequence ligated to the Luminesensor repressible recA408 promoter.
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CheZ coding sequence was placed under RecA408 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K819002 BBa_K819002]).
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===Source===
 
===Source===
  
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CheZ was extracted from E.Coli strain MG1655 by colony PCR.<br /> RecA promoter was from [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819002 BBa_K819002].
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CheZ was extracted from E.Coli strain MG1655 by colony PCR.<br /> RecA408 promoter was from [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819002 BBa_K819002].
  
 
===References===
 
===References===
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1.Scotoc. Kuo and Daniele. Koshland,JR.(1987) Roles of che Y and cheZ Gene Products in Controlling Flagellar Rotation in Bacterial Chemotaxis of Escherichia coli. J. Bacteriol., 3:1307:1313<br />
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2.M. Germana Sanna and Melvin I. Simon(1996) In Vivo and In Vitro Characterization of Escherichia coli Protein CheZ Gain- and Loss-of-Function Mutants. J. Bacteriol., 178: 6275: 6280<br />

Latest revision as of 09:34, 26 September 2012

Luminesensor repressible RecA408 promoter+CheZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CheZ coding sequence was placed under RecA408 promoter (BBa_K819002).

Source

CheZ was extracted from E.Coli strain MG1655 by colony PCR.
RecA408 promoter was from BBa_K819002.

References

1.Scotoc. Kuo and Daniele. Koshland,JR.(1987) Roles of che Y and cheZ Gene Products in Controlling Flagellar Rotation in Bacterial Chemotaxis of Escherichia coli. J. Bacteriol., 3:1307:1313
2.M. Germana Sanna and Melvin I. Simon(1996) In Vivo and In Vitro Characterization of Escherichia coli Protein CheZ Gain- and Loss-of-Function Mutants. J. Bacteriol., 178: 6275: 6280