Difference between revisions of "Part:BBa K929004:Design"

 
 
(7 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K929004 short</partinfo>
 
<partinfo>BBa_K929004 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
We used RFC 25 to combine modified_AID (BBa_929001) and eGFP (BBa_K404316)
+
[[Image:UP_12_designinfo_BBa_K929004.png|center|thumb|600px]]
−
 
+
The modified AID (BBa_929001) is in RFC 10 and has an aditional AgeI restriction site. Therefore it can be fused with RFC 25 parts at the C-terminus. We cut modified AID (BBa_929001)with AgeI and PstI and eGFP (BBa_K404316) with NgoMIV and PstI and ligatet the fragments. The resulting Biobrick ([https://parts.igem.org/part:BBa_K929004 BBa_K929004]) is in RFC 10 and has also an aditional AgeI restriction site in front of the
−
 
+
stop codon taa. Therefore further fusion(C-terminal of [https://parts.igem.org/part:BBa_K929004 BBa_K929004]) with RFC 25 parts is possible.
  
 
===Source===
 
===Source===

Latest revision as of 08:54, 26 September 2012

modified AID+eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1296
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 108
    Illegal BsaI.rc site found at 1225
    Illegal SapI site found at 209


Design Notes

UP 12 designinfo BBa K929004.png

The modified AID (BBa_929001) is in RFC 10 and has an aditional AgeI restriction site. Therefore it can be fused with RFC 25 parts at the C-terminus. We cut modified AID (BBa_929001)with AgeI and PstI and eGFP (BBa_K404316) with NgoMIV and PstI and ligatet the fragments. The resulting Biobrick (BBa_K929004) is in RFC 10 and has also an aditional AgeI restriction site in front of the stop codon taa. Therefore further fusion(C-terminal of BBa_K929004) with RFC 25 parts is possible.

Source

This is a composite part from the existing parts: modified_AID (BBa_929001) and eGFP (BBa_K404316). We used RFC 25 enzymes to create this part as a fusion of modified AID and eGFP (at the C-terminus of mod_AID).

References