Difference between revisions of "Part:BBa K823044"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K823044 short</partinfo> | <partinfo>BBa_K823044 short</partinfo> | ||
| − | MazF is a bacterial toxin from E.coli and degrades mRNA | + | MazF is a bacterial toxin from ''E. coli'' and degrades mRNA |
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| + | [[Image:SuicideSwitchgrafik.jpg|600px]] | ||
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| + | Our team used this part in the combination with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823048 PspoIVB]/[https://parts.igem.org/wiki/index.php?title=Part:BBa_K823042 PsspK],[https://parts.igem.org/Part:BBa_K823043 ECF41] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823044 MazF] in the [http://2012.igem.org/Team:LMU-Munich/Germination_Stop#Suicide_switch <b>Suicide</b> switch]. | ||
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| + | Sigma G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 08:19, 26 September 2012
MazF a bacterial toxin
MazF is a bacterial toxin from E. coli and degrades mRNA
Our team used this part in the combination with PspoIVB/PsspK,ECF41 and MazF in the [http://2012.igem.org/Team:LMU-Munich/Germination_Stop#Suicide_switch Suicide switch].
Sigma G, the last sigma factor in the forespore, activates PspoIVB, producing ECF41 during Sporulation. This then activates the PydfG promoter producing MazF, theoretically killing cells after germination.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]