Difference between revisions of "Part:BBa K782068"

Revision as of 07:53, 26 September 2012

4x[NicTAL]+4x[TALD] operator

• TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).

Introduction

Our construct contains four specific binding sites for NicTAL12 and TALD.

Single binding sequence for NicTAL12 is: TCTATCAATGATAGA

Single binding sequence for TALD is: TCGTCCAATAGCTTCTC

Figure 1. Shematic representation of four consecutive specific binding sites for NicTAL12 and TALD.

Characterization

Results: Specific TAL binding sites were further characterized with different reporter constructs.

• Binding sites for TAL effectors were ordered from IDT.

References

Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698

Sequence and Features