Difference between revisions of "Part:BBa K907001"

Revision as of 07:39, 26 September 2012

Mycobacterium Phage Bxb1 gp47, DNA excisionase

KAIST DNA recombination via Bxb1 Int, Xis.png

This part is a protein coding part which encodes DNA excisionase of Mycobacterium phage Bxb1. The Bxb1 excisionase is a DNA recombinase, more precisely a member of serine integrase family. It forms complex with Bxb1 integrase. The complex recognizes specific sequences, called attL and attR, and then catalyzes DNA recombination that is exact reverse reaction done by integrase alone.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 [[Image: KAIST_DNA_recombination_via_Bxb1_Int,_Xis.png |center|450px]]
 This part is a protein coding part which encodes '''DNA excisionase''' of ''Mycobacterium phage Bxb1''.
-The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called ''attB'' and ''attP'', and then integrates, inverts, or excises dsDNA depending on the orientation of recognition sequences.
+The Bxb1 excisionase is a DNA recombinase, more precisely a member of serine integrase family. It forms complex with Bxb1 integrase. The complex recognizes specific sequences, called ''attL'' and ''attR'', and then catalyzes DNA recombination that is exact reverse reaction done by integrase alone.
-We used this integrase to invert specific sequence in plasmid. This protein is well expressed in ''E.coli(strain MG1655)''.
-When it inverts DNA, the ''attB'' and ''attP'' sequences are changed into'' attL'' and ''attR'', as other DNA recombinases do. Another protein called Bxb1 excisionase forms complex with integrase and this complex flips inverted DNA back into original sequence by regenerating ''attB'' and ''attP'' sequences.
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