Difference between revisions of "Part:BBa K819009"
 
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<partinfo>BBa_K819009 short</partinfo>
 
<partinfo>BBa_K819009 short</partinfo>
  
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CheZ is dephosphorylase of CheY in chemotaxis pathway. CheZ ligated to a weak promoter. This part express CheZ protein weakly and will induce weak mobility in delta CheZ E.coli strain.
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CheZ is a significant protein in <i>E.coli</i> chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.<br />
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CheZ was placed under a weak promoter [https://parts.igem.org/Part:BBa_J23112 BBa_J23112]. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain.
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<br />The photo below shows CheZ's influence on cell motility
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<html>
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<img src="https://static.igem.org/mediawiki/parts/1/16/Peking2012_part_CheZ_K819009.png" style="width:500px;margin-left:10%;"/>
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</html>
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<p>Figure 1. <i>E.coli</i> was grown on semi-solid medium. a)MG1655 strain with cheZ gene deleted. <b>b)cheZ gene under weak promoter BBa_J23112 (this part)</b>. c)cheZ gene under medium promoter BBa_J23113 ([https://parts.igem.org/Part:BBa_K819010 BBa_K819010]) d)wild type MG1655 strain</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 07:30, 26 September 2012

Weak cheZ generator

CheZ is a significant protein in E.coli chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.
CheZ was placed under a weak promoter BBa_J23112. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain.
The photo below shows CheZ's influence on cell motility

Figure 1. E.coli was grown on semi-solid medium. a)MG1655 strain with cheZ gene deleted. b)cheZ gene under weak promoter BBa_J23112 (this part). c)cheZ gene under medium promoter BBa_J23113 (BBa_K819010) d)wild type MG1655 strain


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]