Difference between revisions of "Part:BBa K838002"
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To use the melanopsin pathway, you also need to clone any gene you want to express into the mammalian vector [http://www.promega.com/resources/protocols/product-information-sheets/a/pgl430-vector-protocol/ pGL4.30].
 
To use the melanopsin pathway, you also need to clone any gene you want to express into the mammalian vector [http://www.promega.com/resources/protocols/product-information-sheets/a/pgl430-vector-protocol/ pGL4.30].
  
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Co-transfect the combination of photoreceptive protein and readout construct of your choice in mammalian cells!
 
Co-transfect the combination of photoreceptive protein and readout construct of your choice in mammalian cells!
  
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[[File:Team_EPF_Lausanne_complexswitch.png|400px]]
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[[Image:Team_EPF_Lausanne_complexswitch.png|400px]]
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Revision as of 07:17, 26 September 2012

Melanopsin

Melanopsin is a photosensitive protein etc etc


==

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 282
    Illegal PstI site found at 853
    Illegal PstI site found at 1311
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 282
    Illegal PstI site found at 853
    Illegal PstI site found at 1311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1369
    Illegal BamHI site found at 45
    Illegal BamHI site found at 541
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 282
    Illegal PstI site found at 853
    Illegal PstI site found at 1311
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 282
    Illegal PstI site found at 853
    Illegal PstI site found at 1311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 711

How to use

1) Obtain the parts: To implement a light activated gene switch using this protein, all one needs is this part and any gene to express in a mammalian cell.

2) Cloning:

First clone the part into a mammalian expression vector such as [http://products.invitrogen.com/ivgn/product/V79020?ICID=search-product pcDNA3.1(+)] or [http://products.invitrogen.com/ivgn/product/V04450 pCEP4]. These are the two main expression vectors we used. To use the melanopsin pathway, you also need to clone any gene you want to express into the mammalian vector [http://www.promega.com/resources/protocols/product-information-sheets/a/pgl430-vector-protocol/ pGL4.30].

Team-EPF-Lausanne pNFAT.png


3) Transfection Co-transfect the combination of photoreceptive protein and readout construct of your choice in mammalian cells!

Team EPF Lausanne complexswitch.png