Difference between revisions of "Part:BBa K819009"
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CheZ is a significant protein in <i>E.coli</i> chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.<br /> | CheZ is a significant protein in <i>E.coli</i> chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.<br /> | ||
CheZ was placed under a weak promoter [https://parts.igem.org/Part:BBa_J23112 BBa_J23112]. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain. | CheZ was placed under a weak promoter [https://parts.igem.org/Part:BBa_J23112 BBa_J23112]. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain. | ||
| + | <br />The photo below shows CheZ's influence on cell motility | ||
<html> | <html> | ||
<img src="https://static.igem.org/mediawiki/parts/f/fb/Peking2012_part_CheZ.png" style="width:500px;margin-left:10%;"/> | <img src="https://static.igem.org/mediawiki/parts/f/fb/Peking2012_part_CheZ.png" style="width:500px;margin-left:10%;"/> | ||
| − | <p>Figure 1. </p> | + | <p>Figure 1. <i>E.coli</i> was grown on semi-solid medium. a)MG1655 strain with cheZ gene deleted. b)cheZ gene under weak promoter BBa_J23112 (this part). c)cheZ gene under medium promoter BBa_J23113 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K819010 BBa_K819010]) d)wild type MG1655 strain </p> |
</html> | </html> | ||
<br /> | <br /> | ||
Revision as of 07:10, 26 September 2012
Weak cheZ generator
CheZ is a significant protein in E.coli chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.
CheZ was placed under a weak promoter BBa_J23112. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain.
The photo below shows CheZ's influence on cell motility
Figure 1. E.coli was grown on semi-solid medium. a)MG1655 strain with cheZ gene deleted. b)cheZ gene under weak promoter BBa_J23112 (this part). c)cheZ gene under medium promoter BBa_J23113 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K819010 BBa_K819010]) d)wild type MG1655 strain
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]