Difference between revisions of "Part:BBa K325909:Experience"

[http://2012.igem.org/Team:Peking Peking 2012 iGEM team]

We successfully constructed light communication system using this part-luxbrick, and it was the very first time that cells were able to response to very dim bio-luminesence emitted from luxbrick.
Also we made supplementary characterization of it. See our project of light communication, please click [http://2012.igem.org/Team:Peking/Project/Communication here]

[http://2012.igem.org/Team:Fudan_Lux 2012iGEM team Fudan_Lux]

Growth curve of K325909

In 2012 iGEM competition we measured growth curve of K325909 in a E.coli strain dH5α.

The curve shows that induced by the arabinose, the bacteria growth

Check the RAW data of measurement.

[http://2012.igem.org/Team:Peking/Project/Communication Peking 2012 iGEM team]

We successfully constructed light communication system using this part-luxbrick, and we made supplementary characterization of it.
Spectrum
We measured the spectrum of luminescence produced by luxbrick. As shown in the spectrum below, the luminescence has a maximum intensity at 485nm.

Figure 1. TOP10 cells harboring the luxbrick were cultured in LB medium and induced with L-arabinose at 10^-3M. 10 hours after induction, the glowing cells were measured for spectrum using SHIMADZU RF5301PC Spectrofluorophotometer. (Peking iGEM 2012)

Time course-video
We showed the time course of light emitting process of TOP10 cells transformed with luxbrick in a more visualized way, which is a short video. In the movie, the cells begin to glow 9 hours after induction, and the entire visible glowing process lasts about 10 hours. (Peking iGEM 2012)

See the video, please click [http://www.youtube.com/watch?v=fnhe1MKIO9Q&feature=youtu.be here].
(If you can't see the video above, please click [http://v.youku.com/v_show/id_XNDU0NDU0NDcy.html here])