Difference between revisions of "Part:BBa K750106"
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<partinfo>BBa_K750106 short</partinfo> | <partinfo>BBa_K750106 short</partinfo> | ||
| − | This part contains promoter | + | This part contains promoter ''pBAD'', RBS of 0.3 strength, cI(lva) protein and double terminator. When arabinose is added, the promoter ''pBAD'' will be activated and cI(lva) protein will be produced. cI protein can activated promoter ''Pci'', and repress the production of reporter gene behind it. In our project, sometimes we need the cell can be switched from light to dark, so we designed ''PciGLT''(BBa_K750103), a GFP expression system which can be repressed by cI protein. And this part can provide cI protein for ''PcIGLT''. |
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Latest revision as of 01:44, 26 September 2012
pBADcIT-0.3:cI protein expression system activated by arabinose(RBS strength:0.3)
This part contains promoter pBAD, RBS of 0.3 strength, cI(lva) protein and double terminator. When arabinose is added, the promoter pBAD will be activated and cI(lva) protein will be produced. cI protein can activated promoter Pci, and repress the production of reporter gene behind it. In our project, sometimes we need the cell can be switched from light to dark, so we designed PciGLT(BBa_K750103), a GFP expression system which can be repressed by cI protein. And this part can provide cI protein for PcIGLT.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]