Difference between revisions of "Part:BBa K750114"
m |
|||
Line 2: | Line 2: | ||
<partinfo>BBa_K750114 short</partinfo> | <partinfo>BBa_K750114 short</partinfo> | ||
− | This part contains PtetcIT(BBa_K750102) and PcIGLT(BBa_K750103). Atc can activate the promoter pBAD and start up expression of cI protein. Protein cI will activated promoter PcI to repress the expression of GFP(lva). We use this part to make a bright cell turn to dark, to meet demand of number switching. | + | This part contains ''PtetcIT''(BBa_K750102) and ''PcIGLT''(BBa_K750103). Atc can activate the promoter ''pBAD'' and start up expression of cI protein. Protein cI will activated promoter ''PcI'' to repress the expression of GFP(lva). We use this part to make a bright cell turn to dark, to meet demand of number switching. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 01:30, 26 September 2012
PtetcIT-PcIGLT: atc->cI->GFP(lva)
This part contains PtetcIT(BBa_K750102) and PcIGLT(BBa_K750103). Atc can activate the promoter pBAD and start up expression of cI protein. Protein cI will activated promoter PcI to repress the expression of GFP(lva). We use this part to make a bright cell turn to dark, to meet demand of number switching.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2610