Difference between revisions of "Part:BBa K934025"
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<partinfo>BBa_K934025 short</partinfo> | <partinfo>BBa_K934025 short</partinfo> | ||
| − | We constructed this part by ligating Plux/tet hybrid promoter ([https://parts.igem.org/Part:BBa_K934025 BBa_K934025]) to the upstream of promoterless GFP generator (BBa_I13504). | + | We constructed this part by ligating Plux/tet hybrid promoter ([https://parts.igem.org/Part:BBa_K934025 BBa_K934025]) to the upstream of promoterless GFP generator ([https://parts.igem.org/Part:BBa_K934025 BBa_I13504]). |
[[Image:Pluxtet_assay.png|thumb|center|500px|]] | [[Image:Pluxtet_assay.png|thumb|center|500px|]] | ||
Revision as of 00:32, 26 September 2012
Plux/tet-GFP
We constructed this part by ligating Plux/tet hybrid promoter (BBa_K934025) to the upstream of promoterless GFP generator (BBa_I13504).
In the presence of both inducers, the culture showed about 500-fold higher fluorescence intensity than that in the absence of both inducers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 751