Difference between revisions of "Part:BBa K864401"
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aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone ''Actinia equina''. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.
 
aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone ''Actinia equina''. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.
  
 +
===Source===
 +
The protein was first extracted and characterized in (Shkrob et. al., 2005) under the name aeCP597. This version is codon optimized for E.''coli''
 +
 +
Shkrob et. al. reported that the sum of the following substitutions compared to wild-type resulted in a far-red-shifted but radiation sensitive fluorescent protein:
 +
 +
K6T<br>
 +
K7E<br>
 +
Y101S<br>
 +
C143S<br>
 +
M146I<br>
 +
S158A<br>
 +
 +
These following substitutions induced a more thermostable and photostable product than the above product
 +
 +
S3P<br>
 +
V19I<br>
 +
Y101S<br>
 +
M146I<br>
 +
N168D<br>
  
  

Revision as of 00:18, 26 September 2012

aeBlue blue chromoprotein

aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.

Source

The protein was first extracted and characterized in (Shkrob et. al., 2005) under the name aeCP597. This version is codon optimized for E.coli

Shkrob et. al. reported that the sum of the following substitutions compared to wild-type resulted in a far-red-shifted but radiation sensitive fluorescent protein:

K6T
K7E
Y101S
C143S
M146I
S158A

These following substitutions induced a more thermostable and photostable product than the above product

S3P
V19I
Y101S
M146I
N168D


References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/]Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]