Difference between revisions of "Part:BBa K864440"
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You can use this natural scaffold to make your own sRNA, inhibiting translation on a gene of your choice. | You can use this natural scaffold to make your own sRNA, inhibiting translation on a gene of your choice. | ||
The sRNA is coupled to a strong promotor, J23101 | The sRNA is coupled to a strong promotor, J23101 | ||
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| + | ===Reference=== | ||
| + | Engineering Artificial Small RNAs for Conditional Gene Silencing in Escherichia coli | ||
| + | Vandana Sharma, Asami Yamamura, and Yohei Yokobayashi | ||
| + | ACS Synthetic Biology 2012 1 (1), 6-13 | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 00:09, 26 September 2012
Small RNA spot42
The natural smallRNA spot42 taken from the E-coli MG1655. SmallRNA is belived to interact in many metabolic pathways. The spot42 scaffold interacts with HFQ-proteins. It is in many cases shown to bind into the rbs area of the mRNA and thereby blocking translation initiation. ( V.Sharma, A.Yamamura Y.Yokobayashi, 2011 )
We wanted to use this sRNA for gene downregulation on translational level, in this case block the ribosome from binding to the rbs area.
You can use this natural scaffold to make your own sRNA, inhibiting translation on a gene of your choice. The sRNA is coupled to a strong promotor, J23101
Reference
Engineering Artificial Small RNAs for Conditional Gene Silencing in Escherichia coli Vandana Sharma, Asami Yamamura, and Yohei Yokobayashi ACS Synthetic Biology 2012 1 (1), 6-13
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]