Difference between revisions of "Part:BBa K864403"

Latest revision as of 23:57, 25 September 2012

J23110-B0034-aeBlue (chromoprotein) (E.coli optimized)

This part is a coding device for the chromoprotein aeBlue (BBa_K864401) by means of promoter BBa_J23110 and the RBS BBa_B0034.

aeBlue (more info on BBa_K864401) is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/]Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K864403 short</partinfo>
-aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.
+This part is a coding device for the chromoprotein aeBlue (<partinfo>BBa_K864401</partinfo>) by means of promoter <partinfo>BBa_J23110</partinfo> and the RBS <partinfo>BBa_B0034</partinfo>.
+aeBlue (more info on <partinfo>BBa_K864401</partinfo>) is a blue chromoprotein extracted from the basal disk of a beadlet anemone ''Actinia equina''. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.
+[[Image:IMG 4371a.jpg|400px]]
+[[Image:AeBlue_onplate.jpg|400px]]
-This part is a functionally active variant of BBa_K864401.
+===References===
+[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/]Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part is useful for visual screening and for example be used instead of can be used as
 <!-- -->