Difference between revisions of "Part:BBa K750106:Design"
 
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===Design Notes===
 
===Design Notes===
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Lasr year, we use IPTG to induct the production of LuxI protein and LuxR protein. While this year we hope that there are an obvious process from dark to light. Because of the strong basal expression of promoter LuxPR(BBa_R0062), we have to change the promoter LuxPR(BBa_R0062) to Pbad(BBa_K206000).
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This part is designed to be a NOT gate for our poject.
  
 
===Source===
 
===Source===
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We built this part by using the biobricks from the dna distribution plates 2012.
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We built this part by biobricks from the DNA distribution kit plates 2012. The inspiration of design this part comes from further research of the iccdB project we designed last year.
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===References===
 
===References===
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[1]W.Claiborne Fuqua, S.C.W., E. Peter Greenberg, Quorum Sensing in Bacteria: the LuxR-LuxI Family of Cell Density-Responsive Transcriptional Regulatorst. JOURNAL OF BACTERIOLOGY, 1994. 176(2): p. 269-275.<br><br>
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[1]Tamsir, A., J.J. Tabor, and C.A. Voigt, Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. Nature, 2011. 469(7329): p. 212-5
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[2]You, L., et al., Programmed population control by cell-cell communication and regulated killing. Nature, 2004. 428(6985): p. 868-71.<br><br>
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[3]Alon, U., Network motifs: theory and experimental approaches. Nat Rev Genet, 2007. 8(6): p. 450-61.<br><br>
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[4]Mangan, S., et al., The incoherent feed-forward loop accelerates the response-time of the gal system of Escherichia coli. J Mol Biol, 2006. 356(5): p. 1073-81.<br><br>
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[5]Camas, F.M., J. Blazquez, and J.F. Poyatos, Autogenous and nonautogenous control of response in a genetic network. Proc Natl Acad Sci U S A, 2006. 103(34): p. 12718-23.<br><br>
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[6]http://2011.igem.org/Team:XMU-China<br><br>
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Latest revision as of 23:06, 25 September 2012

pBADcIT-0.3:cI protein expression system activated by arabinose(RBS strength:0.3)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed to be a NOT gate for our poject.

Source

We built this part by using the biobricks from the dna distribution plates 2012.

References

[1]Tamsir, A., J.J. Tabor, and C.A. Voigt, Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. Nature, 2011. 469(7329): p. 212-5