Difference between revisions of "Part:BBa K808025"

(Usage and Biology)
(Usage and Biology)
Line 12: Line 12:
 
* [http://2012.igem.org/Team:TU_Darmstadt/Modeling_Docking#Docking Docking Simulations]
 
* [http://2012.igem.org/Team:TU_Darmstadt/Modeling_Docking#Docking Docking Simulations]
  
Our Material Science Lab performed
+
Our [http://2012.igem.org/Team:TU_Darmstadt/Project/Material_Science Material Science Lab] performed
 
*[http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Material_Science#Surface_analysis_of_polyethylene_terephthalate_with_atomic_force_microscopy_.28AFM.29 Surface analysis of polyethylene terephthalat with atomic force microscopy]
 
*[http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Material_Science#Surface_analysis_of_polyethylene_terephthalate_with_atomic_force_microscopy_.28AFM.29 Surface analysis of polyethylene terephthalat with atomic force microscopy]
 +
 +
Our [http://2012.igem.org/Team:TU_Darmstadt/Project/Degradation Team Degradation]
 +
* [http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Degradation#Enzyme_Activity_Assays Enzyme activity assay] using a [http://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#pNP-Assay pNP-Assay]
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 22:34, 25 September 2012

FsC: Cutinase PET cleaving enzyme

A cutinase is a cuticula degrading hydrolase from the fungus Fusarium solani pisi. It shows activity towards PET.


Usage and Biology

The characterization of FsC occured in 3 different ways.

Our [http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Simulation Simulation Lab] performed

  • [http://2012.igem.org/Team:TU_Darmstadt/Modeling_Docking#Docking Docking Simulations]

Our [http://2012.igem.org/Team:TU_Darmstadt/Project/Material_Science Material Science Lab] performed

  • [http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Material_Science#Surface_analysis_of_polyethylene_terephthalate_with_atomic_force_microscopy_.28AFM.29 Surface analysis of polyethylene terephthalat with atomic force microscopy]

Our [http://2012.igem.org/Team:TU_Darmstadt/Project/Degradation Team Degradation]

  • [http://2012.igem.org/Team:TU_Darmstadt/Labjournal/Degradation#Enzyme_Activity_Assays Enzyme activity assay] using a [http://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#pNP-Assay pNP-Assay]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 301