Difference between revisions of "Part:BBa K911003:Design"

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===Design Notes===
 
===Design Notes===
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Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter. In this case, lacZ  was used as our reporter.
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Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter. In this case, lacZ  was used as our reporter. We have included the lysine promoter as part of this biobrick as a reliably constitutively active promoter, ensuring that the rate of transcription of downstream genes should only be affected by the rate of transcriptional attenuation by the fluoride riboswitch.
  
 
===Source===
 
===Source===
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*Bacillus Cereus Genome
 
*Bacillus Cereus Genome
  
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*Many thanks to the Breaker lab at Yale University, who provided us with the original lacZ reporter construct, as well as a strain of bacillus subtilis that had had its fluoride riboswitch knocked out.
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*Many thanks to the Breaker lab at Yale University, who provided us with the original lacZ reporter construct, as well as a knockout strain of bacillus subtilis.
  
 
===References===
 
===References===
  
 
* Jenny L. Baker et al., Widespread Genetic Switches and Toxicity Resistance Proteins for Fluoride, Science (2012) vol. 335 pp. 233 - 235.
 
* Jenny L. Baker et al., Widespread Genetic Switches and Toxicity Resistance Proteins for Fluoride, Science (2012) vol. 335 pp. 233 - 235.

Latest revision as of 22:07, 25 September 2012

Fluoride Sensitive Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 26
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter. In this case, lacZ was used as our reporter. We have included the lysine promoter as part of this biobrick as a reliably constitutively active promoter, ensuring that the rate of transcription of downstream genes should only be affected by the rate of transcriptional attenuation by the fluoride riboswitch.

Source

  • Bacillus Cereus Genome
  • Many thanks to the Breaker lab at Yale University, who provided us with the original lacZ reporter construct, as well as a knockout strain of bacillus subtilis.

References

  • Jenny L. Baker et al., Widespread Genetic Switches and Toxicity Resistance Proteins for Fluoride, Science (2012) vol. 335 pp. 233 - 235.