Difference between revisions of "Part:BBa K911003:Design"

Revision as of 22:05, 25 September 2012

Fluoride Sensitive Riboswitch

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 26
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter. In this case, lacZ was used as our reporter. We have included the lysine promotor as part of this biobrick as a reliably constitutively active promotor, ensuring that the rate of transcription of downstream genes should only be affected by the rate of transcriptional attenuation by the fluoride riboswitch.

Source

Bacillus Cereus Genome

Many thanks to the Breaker lab at Yale University, who provided us with the original lacZ reporter construct, as well as a knockout strain of bacillus subtilis.

References

Jenny L. Baker et al., Widespread Genetic Switches and Toxicity Resistance Proteins for Fluoride, Science (2012) vol. 335 pp. 233 - 235.

@@ Line 6: / Line 6: @@
 ===Design Notes===
-Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter. In this case, lacZ  was used as our reporter.
+Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter. In this case, lacZ  was used as our reporter. We have included the lysine promotor as part of this biobrick as a reliably constitutively active promotor, ensuring that the rate of transcription of downstream genes should only be affected by the rate of transcriptional attenuation by the fluoride riboswitch.
 ===Source===