Difference between revisions of "Part:BBa K863006:Design"

(→‎Source)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix the rev primer. <br>
+
Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix in the rev primer. <br>
 
fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGATGCAACGTCGTGATTTCTT-3' <br>
 
fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGATGCAACGTCGTGATTTCTT-3' <br>
 
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTATACCGTAAACCCTAACA-3'
 
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTATACCGTAAACCCTAACA-3'

Revision as of 21:53, 25 September 2012

ecol laccase from E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGATGCAACGTCGTGATTTCTT-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTATACCGTAAACCCTAACA-3'

Source

The gene sequence was isolated from E. coli BL21(DE3).

References