Difference between revisions of "Part:BBa K808027"
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<partinfo>BBa_K808027 short</partinfo> | <partinfo>BBa_K808027 short</partinfo> | ||
− | + | EstA is a membrane bound but inactive esterase from ''Pseudomonas aeruginosa''. It can be used (unlike https://parts.igem.org/Part:BBa_K633001 BBa_K633001) for the cell surface expression of enzymes. Similiar to OmpA it allows to perform bacterial display of e.g. hydrolases like it has already been shown by Becker, Theile, Heppeler & Michalczyk, 2005. We used EstA to create our chimeric protein [https://parts.igem.org/Part:BBa_K808030 BBa_K808030](Figure 1). | |
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+ | [[Image:pnb_EstA_ohne_mem.png|400px|center|thumb|Figure 1:[https://parts.igem.org/Part:BBa_K808030 BBa_K808030] EstA (red & grey) anchoring a second protein (pNB-Est13 in yellow) into a biological membrane]] | ||
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Revision as of 21:47, 25 September 2012
EstA : inactive fusion protein for bacterial surface expression
EstA is a membrane bound but inactive esterase from Pseudomonas aeruginosa. It can be used (unlike https://parts.igem.org/Part:BBa_K633001 BBa_K633001) for the cell surface expression of enzymes. Similiar to OmpA it allows to perform bacterial display of e.g. hydrolases like it has already been shown by Becker, Theile, Heppeler & Michalczyk, 2005. We used EstA to create our chimeric protein BBa_K808030(Figure 1).
![](/wiki/images/4/45/Pnb_EstA_ohne_mem.png)
Figure 1:BBa_K808030 EstA (red & grey) anchoring a second protein (pNB-Est13 in yellow) into a biological membrane
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 97
Illegal NgoMIV site found at 235
Illegal NgoMIV site found at 775
Illegal NgoMIV site found at 1144 - 1000COMPATIBLE WITH RFC[1000]