Difference between revisions of "Part:BBa K782082"

 
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<partinfo>BBa_K782082 short</partinfo>
 
<partinfo>BBa_K782082 short</partinfo>
  
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long
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==Introduction==
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Our construct contain twelve binding sites for [https://parts.igem.org/Part:BBa_K782011 NicTAL12]([https://parts.igem.org/Part:BBa_K782003 sequence]) that are cloned upstream of CMV promoter. Upstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
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[[Image:Nicbfp2.png‎‎]]
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'''Figure 1:''' Schematic representation of the construct.
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==Characterization==
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Results:
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BFP was obtained from Evrogen.
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Revision as of 21:38, 25 September 2012

12x[NicTAL] operator_CMV promoter_2xBFP:NLS


Introduction

Our construct contain twelve binding sites for NicTAL12(sequence) that are cloned upstream of CMV promoter. Upstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

Nicbfp2.png

Figure 1: Schematic representation of the construct.


Characterization

Results:

BFP was obtained from Evrogen.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 16
    Illegal AgeI site found at 248
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1139
    Illegal BsaI.rc site found at 1795
    Illegal BsaI.rc site found at 2500