Difference between revisions of "Part:BBa K782077"
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Our construct contain twelve binding sites for [NicTAL12] that are cloned downstream of CMV promoter. Upstream of CMV is Blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm. | Our construct contain twelve binding sites for [NicTAL12] that are cloned downstream of CMV promoter. Upstream of CMV is Blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm. | ||
| − | [[Image:Nicbfp. | + | [[Image:Nicbfp.png]] |
'''Figure 1:''' Schematic representation of the construct. | '''Figure 1:''' Schematic representation of the construct. | ||
Revision as of 20:59, 25 September 2012
12x[NicTAL] operator_CMV promoter_BFP
Introduction
Our construct contain twelve binding sites for [NicTAL12] that are cloned downstream of CMV promoter. Upstream of CMV is Blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
Figure 1: Schematic representation of the construct.
Characterization
Results:
BFP was obtained from Evrogen.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 464
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 16
Illegal AgeI site found at 248 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1140
Illegal BsaI.rc site found at 1796