Difference between revisions of "Part:BBa K782076"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K782076 short</partinfo> | <partinfo>BBa_K782076 short</partinfo> | ||
| − | + | ==Introduction== | |
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| + | Our construct contain [https://parts.igem.org/Part:BBa_K782072 TALD binding sites] that are cloned downstream of CMV promoter. Upstream of CMV is Blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm. | ||
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| + | [[Image:dbfp.png]] | ||
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| + | '''Figure 1:''' Schematic representation of the construct. | ||
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| + | ==Characterization== | ||
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| + | Results: | ||
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| + | BFP was obtained from Evrogen. | ||
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Revision as of 20:30, 25 September 2012
12x[TALD] operator_CMV promoter_BFP
Introduction
Our construct contain TALD binding sites that are cloned downstream of CMV promoter. Upstream of CMV is Blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
Figure 1: Schematic representation of the construct.
Characterization
Results:
BFP was obtained from Evrogen.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 465
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 214
Illegal AgeI site found at 446 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1141
Illegal BsaI.rc site found at 1797