Difference between revisions of "Part:BBa K907002"
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<br> 1. RBSs: [https://parts.igem.org/Part:BBa_B0034 BBa_B0034], upsteam one is reversed | <br> 1. RBSs: [https://parts.igem.org/Part:BBa_B0034 BBa_B0034], upsteam one is reversed | ||
<br> 2. Promoter: [https://parts.igem.org/Part:BBa_J23119 BBa_J23119], Bacterial constitutive promoter | <br> 2. Promoter: [https://parts.igem.org/Part:BBa_J23119 BBa_J23119], Bacterial constitutive promoter | ||
| − | <br> 3. att sites: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase | + | <br> 3. att sites: Recognition site for [https://parts.igem.org/Part:BBa_K907000 BBa_K907000](Mycobacteriophage Bxb1 integrase)/excisionase |
<br> We designed this part to generate two different proteins one at a time. | <br> We designed this part to generate two different proteins one at a time. | ||
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<br>[[Image: KAIST_Dual_Phase_protein_generator_inverted.png |left|600px]] | <br>[[Image: KAIST_Dual_Phase_protein_generator_inverted.png |left|600px]] | ||
Initially, this device promotes the transcription and translation of downstream gene due to its promoter orientation. | Initially, this device promotes the transcription and translation of downstream gene due to its promoter orientation. | ||
| − | <br>When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation is reversed. Then this device promotes the transcription and translation of upstream gene(must be designed in reverse orientation at construction step). | + | <br><br>When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation is reversed. Then this device promotes the transcription and translation of upstream gene(must be designed in reverse orientation at construction step). |
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Revision as of 20:20, 25 September 2012
Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS
<Part Description>
This part is composed of 3 elements.
1. RBSs: BBa_B0034, upsteam one is reversed
2. Promoter: BBa_J23119, Bacterial constitutive promoter
3. att sites: Recognition site for BBa_K907000(Mycobacteriophage Bxb1 integrase)/excisionase
We designed this part to generate two different proteins one at a time.
<Application>
Initially, this device promotes the transcription and translation of downstream gene due to its promoter orientation.
When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation is reversed. Then this device promotes the transcription and translation of upstream gene(must be designed in reverse orientation at construction step).
The promoter reversed version is BBa_K907003.
This device is tested by GFP and mRFP attached construct. See BBa_K0907004 and BBa_K0907005.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 75
Illegal NheI site found at 98 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 157
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 22
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 42
Illegal BsaI.rc site found at 125