Difference between revisions of "Part:BBa K907001"
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<partinfo>BBa_K907001 short</partinfo>
 
<partinfo>BBa_K907001 short</partinfo>
  
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This part is the protein coding sequence which encodes DNA integrase of Mycobacterium phage Bxb1.
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The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called attB and attP, and then integrate, invert, or excise depend on orientations of recognition sequences.
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<br>
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We used this integrase to invert specific sequence contained in plasmid. This protein is well expressed in E.coli(strain MG1655).
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[[Image: KAIST_DNA_recombination_via_Bxb1_Int,_Xis.png |center|450px]]
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When it inverts DNA, the attB and attP sequences are changed into attL and attR, as common DNA recombinases do. Another protein called Bxb1 excisionase interacts with integrase and that complex flips back inverted DNA into original sequence regenerating attB and attP sequences.  
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This part is a protein coding part which encodes '''DNA excisionase''' of ''Mycobacterium phage Bxb1''.
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The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called ''attB'' and ''attP'', and then integrates, inverts, or excises dsDNA depending on the orientation of recognition sequences.
 +
We used this integrase to invert specific sequence in plasmid. This protein is well expressed in ''E.coli(strain MG1655)''.
 +
When it inverts DNA, the ''attB'' and ''attP'' sequences are changed into'' attL'' and ''attR'', as other DNA recombinases do. Another protein called Bxb1 excisionase forms complex with integrase and this complex flips inverted DNA back into original sequence by regenerating ''attB'' and ''attP'' sequences.  
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Revision as of 20:20, 25 September 2012

Mycobacterium Phage Bxb1 gp47, DNA excisionase


KAIST DNA recombination via Bxb1 Int, Xis.png

This part is a protein coding part which encodes DNA excisionase of Mycobacterium phage Bxb1. The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called attB and attP, and then integrates, inverts, or excises dsDNA depending on the orientation of recognition sequences. We used this integrase to invert specific sequence in plasmid. This protein is well expressed in E.coli(strain MG1655). When it inverts DNA, the attB and attP sequences are changed into attL and attR, as other DNA recombinases do. Another protein called Bxb1 excisionase forms complex with integrase and this complex flips inverted DNA back into original sequence by regenerating attB and attP sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]