Difference between revisions of "Part:BBa K782068"
Anja Golob (Talk | contribs) |
Anja Golob (Talk | contribs) |
||
| Line 6: | Line 6: | ||
==Introduction== | ==Introduction== | ||
| − | Transcription activation like (TAL) effectors are bacterial plant pathogen transcription factors that bind to DNA by recognizing a specific DNA sequence in which each base pair binds a single tandem repeat in in the TAL DNA-binding domain. A tandem TAL repeat contains 33 to 35 amino acids, where the 12th and the 13th amino acid, called a “repeat variable diresidue” (RVD) are responsible for specific interactions with the corresponding base pair (Scholze and Boch, 2011). All TAL repeats have almost identical sequences, differing only in the RVDs. This modularity of TAL effector binding domains therefore makes them a perfect tool to target specific DNA sequences by designing specific binding domains for a selected TAL effector. We designed four consecutive specific binding sites for | + | Transcription activation like (TAL) effectors are bacterial plant pathogen transcription factors that bind to DNA by recognizing a specific DNA sequence in which each base pair binds a single tandem repeat in in the TAL DNA-binding domain. A tandem TAL repeat contains 33 to 35 amino acids, where the 12th and the 13th amino acid, called a “repeat variable diresidue” (RVD) are responsible for specific interactions with the corresponding base pair (Scholze and Boch, 2011). All TAL repeats have almost identical sequences, differing only in the RVDs. This modularity of TAL effector binding domains therefore makes them a perfect tool to target specific DNA sequences by designing specific binding domains for a selected TAL effector. We designed four consecutive specific binding sites for NicTAL12 and TALD (Figure 1). |
| − | Single binding sequence for | + | Single binding sequence for [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782007 NicTAL12] is: TCTATCAATGATAGA |
| − | Single binding sequence for TALD is: TCGTCCAATAGCTTCTC | + | Single binding sequence for [https://parts.igem.org/wiki/index.php?title=Part:BBa_K782005 TALD] is: TCGTCCAATAGCTTCTC |
Revision as of 19:43, 25 September 2012
4x[NicTAL]+4x[TALD] operator
- TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
Introduction
Transcription activation like (TAL) effectors are bacterial plant pathogen transcription factors that bind to DNA by recognizing a specific DNA sequence in which each base pair binds a single tandem repeat in in the TAL DNA-binding domain. A tandem TAL repeat contains 33 to 35 amino acids, where the 12th and the 13th amino acid, called a “repeat variable diresidue” (RVD) are responsible for specific interactions with the corresponding base pair (Scholze and Boch, 2011). All TAL repeats have almost identical sequences, differing only in the RVDs. This modularity of TAL effector binding domains therefore makes them a perfect tool to target specific DNA sequences by designing specific binding domains for a selected TAL effector. We designed four consecutive specific binding sites for NicTAL12 and TALD (Figure 1).
Single binding sequence for NicTAL12 is: TCTATCAATGATAGA
Single binding sequence for TALD is: TCGTCCAATAGCTTCTC
Figure 1. Shematic representation of four consecutive specific binding sites for NicTAL and TALD.
- Binding sites for TAL effectors were ordered from IDT.
References
Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.
Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 35
Illegal AgeI site found at 235 - 1000COMPATIBLE WITH RFC[1000]