Difference between revisions of "Part:BBa K883701"

Latest revision as of 19:35, 25 September 2012

Figure 1: cross-section view of a E-/ K-coil heterodimer'

GFP with a His-tag fused to a coiled coil

GFP with a His tag at the C-terminus and the E-coil at the N-terminus to use it as reporter for the E- and K-coil [http://2012.igem.org/Team:Wageningen_UR/Coil_system Plug and Apply system]

Usage and Biology

The E-coil present in this reporter protein is one part of the Plug and Apply (PnA) system consisting of a combination of E- and K-coil. These coils consist of α-helical repeats and have a strong affinity to each other due to electrostatic interactions (with K-coil positively charged and E-coil negatively charged).

Using this system one can bind two proteins noncovalently but specifically to each other.

This brick can be used for confirmation of the Plug and Apply system using E- and K-coils as well as for detection of proteins that can be fused to the K-coil.

Figure 2: protein folding prediction of the E-coil attached to a GFP-molecule'

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 710

@@ Line 1: / Line 1: @@
+[[Image:GNC4_homodimer.jpg|800px|thumb|<p align="justify">''Figure 1: cross-section view of a E-/ K-coil heterodimer'</p>]]
 __NOTOC__
 <partinfo>BBa_K883701 short</partinfo>
+GFP with a His tag at the C-terminus and the E-coil at the N-terminus to use it as reporter for the E- and K-coil [http://2012.igem.org/Team:Wageningen_UR/Coil_system Plug and Apply system]
-GFP with a His tag at the C-terminus and the E-coil at the N-terminus to use it as reporter for the E- and K-coil [http://2012.igem.org/Team:Wageningen_UR/Coil_system Plug and Apply sytem]
+===Usage and Biology===
+The E-coil present in this reporter protein is one part of the Plug and Apply (PnA) system consisting of a combination of E- and K-coil. These coils consist of α-helical repeats and have a strong affinity to each other due to electrostatic interactions (with K-coil positively charged and E-coil negatively charged).
+Using this system one can bind two proteins noncovalently but specifically to each other.
+This brick can be used for confirmation of the Plug and Apply system using E- and K-coils as well as for detection of proteins that can be fused to the K-coil.
+[[Image:GFP-coil.png|800px|center|thumb|<p align="justify">''Figure 2: protein folding prediction of the E-coil attached to a GFP-molecule'</p>]]
 <!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->