Difference between revisions of "Part:BBa K792003"
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Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process ''[Derossi 1998]''. They '''can be used to increase the efficiency with which a protein enters a cell'''.
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Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process ''[Jones et al 2005]''. They '''can be used to increase the efficiency with which a protein enters a cell'''.
  
  

Revision as of 19:22, 25 September 2012

Import enhancer - HIV TAT penetratin (trojan peptide)


Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Jones et al 2005]. They can be used to increase the efficiency with which a protein enters a cell.


This part is the penetratin from the HIV TAT protein, and has to be used just before the coding sequence of the peptide that you want to be import enhanced.


This part includes a HindIII restriction site as an extra.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]