Difference between revisions of "Part:BBa K777109:Design"

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===Design Notes===
 
===Design Notes===
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* The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
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* The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via [https://static.igem.org/mediawiki/2012/3/34/Overlap_PCR.pdf overlap-PCR].
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* ''fliC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
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** Fwd: gtttcttcgaattcgcggccgcttctagatggcacaagtcattaatacc
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** Rev: gtttcttcgaattcgcggccgcttctagttaaccctgGagcagagacagaacc <br> (the capitalized "G" induces the mutation for removal of the third PstI site)
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===Source===
 
===Source===

Latest revision as of 18:10, 25 September 2012

fliC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
    Illegal AgeI site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • The fliC gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.

Source

  • The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).

References