Difference between revisions of "Part:BBa K896007"
 
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<partinfo>BBa_K896007 short</partinfo>
 
<partinfo>BBa_K896007 short</partinfo>
  
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A type of respiratory NO3-reductase is a water-soluble, periplasmicallylocated protein known as Nap. We acquire the Nap gene from Desulfovibrio desulfuricans ATCC 27774, the function of Nap is to transfer NO into nitrogen.<p></p>
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Nap is periplasmic nitrate reductase, which is known for the ability to reduce nitrate into nitrite. In microorganisms, similar reductases are often observed. Even E. coli has its own nitrate reductase and can use nitrate as final electron acceptor. Moreover, we acquire the Nap gene from Desulfovibrio desulfuricans ATCC 27774.
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[[Image:Nap cloning.jpg]]
 
[[Image:Nap cloning.jpg]]
  
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The Prokaryotic Nitrate Reductases
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David J. Richardson, Rob J.M. van Spanning and Stuart J. Ferguson
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(David J. Richardson, Rob J.M. van Spanning and Stuart J. Ferguson, The Prokaryotic Nitrate Reductases)
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:05, 25 September 2012

Nap( Nitrate->Nitrite reductase)

Nap is periplasmic nitrate reductase, which is known for the ability to reduce nitrate into nitrite. In microorganisms, similar reductases are often observed. Even E. coli has its own nitrate reductase and can use nitrate as final electron acceptor. Moreover, we acquire the Nap gene from Desulfovibrio desulfuricans ATCC 27774.

Nap cloning.jpg


(David J. Richardson, Rob J.M. van Spanning and Stuart J. Ferguson, The Prokaryotic Nitrate Reductases)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1594
    Illegal PstI site found at 2427
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1594
    Illegal PstI site found at 2427
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1594
    Illegal BamHI site found at 1803
    Illegal XhoI site found at 2361
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1594
    Illegal PstI site found at 2427
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1594
    Illegal PstI site found at 2427
    Illegal NgoMIV site found at 2983
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1342
    Illegal SapI.rc site found at 1903