Difference between revisions of "Part:BBa K934012"
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<partinfo>BBa_K934012 short</partinfo> | <partinfo>BBa_K934012 short</partinfo> | ||
| − | We constructed this part by combining | + | We constructed this part by combining [https://parts.igem.org/Part:BBa_K649000 BBa_K649000] and [https://parts.igem.org/Part:BBa_K081008 BBa_K081008]. |
This part generates LuxI enzyme in the presence of LasR-3OC12HSL complex. | This part generates LuxI enzyme in the presence of LasR-3OC12HSL complex. | ||
| Line 10: | Line 10: | ||
In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI(BBa-K934012) synthesized 3OC6HSL. | In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI(BBa-K934012) synthesized 3OC6HSL. | ||
| − | We improved a previous part Plas-LuxI([https://parts.igem.org/Part:BBa_K266000 BBa_K266000]) | + | We improved a previous part Plas-LuxI ([https://parts.igem.org/Part:BBa_K266000 BBa_K266000]) |
| − | and accomplish a positive feedback system with our new Plux-LasI([https://parts.igem.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]). | + | and accomplish a positive feedback system with our new Plux-LasI ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]). |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 17:59, 25 September 2012
Plas-LuxI
We constructed this part by combining BBa_K649000 and BBa_K081008. This part generates LuxI enzyme in the presence of LasR-3OC12HSL complex.
In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI(BBa-K934012) synthesized 3OC6HSL.
We improved a previous part Plas-LuxI (BBa_K266000)
and accomplish a positive feedback system with our new Plux-LasI (BBa_K934022).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 749
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]