Difference between revisions of "Part:BBa K934022"

Revision as of 17:56, 25 September 2012

Plux-LasI

We constructed this part by combining R0062 and K081016. This part starts generating LasI enzyme in the presence of LuxR-3OC6HSL complex.

In the presence of 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, “Las reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Las reporter cell” is dually regulated by 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, this result shows that Plux-lasI(K934022) synthesized 3OC12HSL.

We accomplished a positive feedback system with our improved part Plux-LasI(https://parts.igem.org/Part:BBa_K934012 BBa_K934012).

For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 745
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 307
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 In the presence of 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, “Las reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Las reporter cell” is dually regulated by 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, this result shows that Plux-lasI(K934022) synthesized 3OC12HSL.
+We accomplished a positive feedback system with our improved part Plux-LasI(https://parts.igem.org/Part:BBa_K934012 BBa_K934012).
 For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki].