Difference between revisions of "Part:BBa K896004"

Revision as of 17:35, 25 September 2012

NosZ-NorCB

Cloning of NosZ+NorCB gene:

NorCB is known as nitric oxide reductase CB complex, and it is responsible for reducing nitric oxide into nitrous oxide. On the other hand, NosZ stands for the structure gene of nitrous-oxide reductase, which conducts the ultimate process of denitrification; that is, reducing nitrous oxides into nitrogen. In addition, both NorCB and NosZ are originated from Pseudomonas aeruginosa PAO1.

We clone NosZ and NorCB and ligate them together. However, since there are PstI enzyme cutting site inside the NorCB, so we clone a new pSB1C3 backbone which contain EcoRI.XbaI.SpeI.SbfI enzyme cutting site.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1845
Illegal PstI site found at 2489
Illegal PstI site found at 3605
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1845
Illegal PstI site found at 2489
Illegal PstI site found at 3605
Illegal NotI site found at 3764
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1517
Illegal BamHI site found at 2658
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1845
Illegal PstI site found at 2489
Illegal PstI site found at 3605
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1845
Illegal PstI site found at 2489
Illegal PstI site found at 3605
Illegal NgoMIV site found at 908
Illegal NgoMIV site found at 1072
Illegal NgoMIV site found at 2684
Illegal NgoMIV site found at 2726
Illegal NgoMIV site found at 3628
Illegal AgeI site found at 282
Illegal AgeI site found at 1381
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1638
Illegal BsaI.rc site found at 667
Illegal BsaI.rc site found at 1312
Illegal BsaI.rc site found at 1940

@@ Line 4: / Line 4: @@
 Cloning of NosZ+NorCB gene:
-NorCB is known as nitric oxide reductase CB complex, and it is responsible for reducing nitric oxide into nitrous oxide. On the other hand, NosZ stands for the structure gene of nitrous-oxide reductase, which conducts the ultimate process of denitrification; that is, reducing nitrous oxides into nitrogen.
+NorCB is known as nitric oxide reductase CB complex, and it is responsible for reducing nitric oxide into nitrous oxide. On the other hand, NosZ stands for the structure gene of nitrous-oxide reductase, which conducts the ultimate process of denitrification; that is, reducing nitrous oxides into nitrogen. In addition, both NorCB and NosZ are originated from  Pseudomonas aeruginosa PAO1.
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