Difference between revisions of "Part:BBa K819017"

Revision as of 16:31, 25 September 2012

Luminesensor repressible SulA408 promoter

SulA promoter in 408 form.
SulA promoter belongs to the SOS regulon family. In E.coli it promotes the transcription of SulA protein. LexA protein in E.coli can bind to SulA promoter, inhibiting the transcription of the genes downstream.
However, with mutations in specific sites which LexA protein will bind to, SulA 408 promoter will not be bound by LexA protein, but is compatible to LexA 408 protein with corresponding mutations in DNA binding site. So a bio-orthogonal promoter is formed because the influence of endogenous LexA protein is excluded.
For example, the optimized luminesensor with a LexA 408 domain (BBa_K819005) constructed by [http://2012.igem.org/Team:Peking Peking 2012 iGEM team] can specifically bind to SulA 408 promoter and therefore inhibit the gene expression (e.g. BBa_K819006 is a testing device containing recA 408 promoter constructed by Peking 2012 iGEM team)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K819017 short</partinfo>
-SulA promoter in 408 form.SulA promoter belongs to the SOS regulon family, and promotes in E.coli transcription of SulA protein. SulA408 promoter is reponsive to our optimized Luminesensor(BBa_K819005).
+SulA promoter in 408 form.<br />
+SulA promoter belongs to the SOS regulon family. In <i>E.coli</i> it promotes the transcription of SulA protein. LexA protein in <i>E.coli</i> can bind to SulA promoter, inhibiting the transcription of the genes downstream.<br />
+However, with mutations in specific sites which LexA protein will bind to, SulA 408 promoter will not be bound by LexA protein, but is compatible to LexA 408 protein with corresponding mutations in DNA binding site. So a bio-orthogonal promoter is formed because the influence of endogenous LexA protein is excluded.<br />
+For example, the optimized luminesensor with a LexA 408 domain ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K819005 BBa_K819005]) constructed by [http://2012.igem.org/Team:Peking Peking 2012 iGEM team] can specifically bind to SulA 408 promoter and therefore inhibit the gene expression (e.g. [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819006 BBa_K819006] is a testing device containing recA 408 promoter constructed by Peking 2012 iGEM team)<br /><br />
 <!-- Add more about the biology of this part here